Abstract: Echinococcosis is an important public health problem in many parts of the world. High prevalence is found in the middle East as well as Arabic North Africa. Among all available serological assays, the use of highly purified antigens improves the sensitivity of these assays and the use of immunoblot is a powerful confirmatory technique. The present study was carried out to assess the prevalence of echinococcosis using ELISA assay as a screening test in some areas of United Arab Emirates and evaluate the sensitivity of purified antigen B (AgB). A total of 1651 serum samples were collected and tested for the presence of anti-Echinococcus antibodies using enzyme linked immnunosorbent assay (ELISA) and Western Blot (WB) analysis as confirmatory test. The prevalence of cystic echinococcosis was 0.6% among the studied population. No significant difference was found neither between genders, nor different nationalities. The sensitivity of purified AgB based ELISA was 90% in comparison to 70% with commercial ELISA kit with reference to the confirmatory Western blot.