Abstract: Human hydatidosis is a public health problem in many parts of the world and improvement in diagnosis of the disease is still being pursued. It is diagnosed with radiological methods complemented by serological tests but cross-reactivity with other parasites represents a big limitation. The aim of the present study was to evaluate 3 different serological techniques: enzyme-linked immunoelectrotransfer blot (EITB), indirect enzyme linked immunosorbant assay (ELISA) and Dot-ELISA using Laminated Layer (LL) antigen prepared from hydatid cysts driven from infected sheep, for diagnosis of human hydatidosis in patients living in Menoufia Governorate, Egypt. The indirect ELISA for the detection of specific IgG4 antibody achieved a sensitivity of 92% and specificity of 96%. Dot-ELISA (enzyme linked immunosorbant assay) employed LL antigen demonstrated sensitivity of 96% and specificity of 98%. The pool of positive hydatid sera recognized strong bands at 66, 55 and 27 kDa. The 27 kDa band showed the highest sensitivity (80%) and specificity (96%) when reacted with individual human sera. So, the Dot-ELISA was the most sensitive serological tool which might be of value in serodiagnosis of human hydatidosis.