Abstract: Neospora spp. are an obligate intracellular protozoan parasite and are recognized as a major cause of abortion in farm animals. Epidemiological and field studies concerning Neospora spp are very much dependent on serology. The objective of the present study was to determine the seroprevalence of Neospora spp. in Arabian horses used for sporting and showing purposes in Riyadh city, Saudi Arabia. In total, 163 serum samples from physically healthy horses were analyzed for anti-Neospora antibodies using the Neospora Modified Agglutination Test (N-MAT). Antibodies to Neospora spp were 39 (23.9%) of the 163 horses with 1:40 serum dilution, where 1:80 serum dilution was applied as significant cut off dilution and the serum positivity was reduced to 28 (17.1%) of the 163 horses. The differences in age and horse sex were not statistically significant (p<0.05). However, this study is the first seroprevalence study to determine Neospora spp antibodies by a Neospora Modified Agglutination Test (N-MAT) in horses from arid areas in Saudi Arabia.