Abstract: A study was conducted to investigate the amelioration potential of trypanosome infection induced alterations in rat serum cholesterol, triglycerides and proteins by hydro-ethanolic extract of Waltheria indica L. The results from the study showed that infection with Trypanosoma brucei brucei resulted in significant (p<0.05) increases in the concentrations of serum triglycerides (hyperglyceridemia), total protein (hyperproteinaemia) and globulin (hyperglobulinaemia) while the levels of cholesterol and albumin were significantly reduced leading to hypocholesterolaemia and hypoalbuminaemia, respectively. However, treatment with the hydro-ethanolic extract of W. indica significantly (p<0.05) ameliorates these infection induced biochemical changes in fashion comparable to the standard trypanocide Berenil®. The corrective action of the plant may revolve around reduction in parasite load coupled with some hepato-protective properties that need further research to ascertain.