Abstract: The aim of the present study was to determine and compare the antibody (Ab) response against Fasciola gigantica in experimentally infected rabbits treated with mirazid or carnosine by ELISA. Six groups of five rabbits each, were examined (negative control, negative treated with carnosine, negative treated with mirazid, infected positive control, infected treated with carnosine and infected treated with mirazid). Each insulted rabbit was infected with 20 Fasciola metacercariae. Anti-Fasciola IgG was weekly evaluated by ELISA, in each animal serum, along the period of experiment (14 weeks). After scarifying the all animals, each liver slices were examined for the determination of flukes burden. Results showed highly significant difference between the numbers of Fasciola worms in liver of infected treated and control positive rabbits. A reduction of 54.5% in worm burden was detected in the carnosine treated group. While, the results of mirazid treated group presented complete eradication of flukes. IgG response in experimentally infected and treated groups, either with carnosine or mirazid, was significantly higher than that of control infected group. Carnosine infected group showed the highest Ab response of all groups. However carnosine action was not as potent as mirazid although it raised the general defence of the animal (Ab) plus moderate eradication effect on the worm. Thus, a combination of both carnosine and mirazid might only be recommended in patients presenting other causes of diminished immunity in addition to Fasciola infection.