Abstract: Background and Objectives: Dyslipidemia and free radical injury are important risk factors in the pathogenesis of stroke. The observations of various researchers on the association between the parameters of lipid profile and malondialdehyde (product of free radical injury) level in patients with stroke are still inconclusive. This study estimated the plasma concentration of Total Cholesterol (TC), Triglyceride (TG), Low Density Lipoprotein-Cholesterol (LDL-Chol), High Density Lipoprotein-Cholesterol (HDL-Chol) and serum level of malondialdehyde (MDA) in patients with stroke. Materials and Methods: A total of 60 patients with stroke, 40 age and sex-matched volunteers were recruited after obtaining their informed consent. Plasma and serum were extracted from whole blood collected from each subject and control. The serum MDA as well as plasma TC, TG, LDL-Chol and HDL-Chol were determined by various methods in the laboratory. Results: The mean values of MDA (11.02 vs. 7.82 nmol mL–1), TG (1.61 vs. 0.84 mmol L–1) and LDL-Chol (2.74 vs. 2.06 mmol L–1) were significantly higher in patients with stroke than the controls (p<0.001). The mean value of HDL-Chol was significantly lower in stroke patients (1.10 mmol L–1) than the controls (1.60 mmol L–1) (p<0.001). However, no significant difference was observed between the mean TC in stroke patients and controls (4.37 vs. 4.16 mmol L–1, p = 0.215). Total cholesterol has a significant positive correlation with LDL-Chol (r = 0.757, p<0.01) and MDA (r = 0.258, p = 0.047) whereas, it has significant negative correlation with HDL-Chol (r = -0.316, p = 0.014). Conclusion: Stroke is shown to be associated with free radical injury as a result of increased serum MDA. Significant positive correlation between MDA and Total Cholesterol (TC) further explains the link between free radical injury and dyslipidaemia in stroke patients.