Abstract: Background and Objective: Teak (Tectona grandis) belonging to family Verbenaceae, is commonly used in Indonesian cuisine as natural food colorant. However, there remains limited information regarding its toxicity effects. The purpose of this study was to examine Tectona grandis leaf extract (TGLF) acute toxicity in Wistar rat animal models through oral route administration. Materials and Methods: Acute oral toxicity of TGLF was carried out in single dose of 2 and 5 g kg–1 b.wt., (dissolved in NaCMC). Animal models were grouped into control (NaCMC 0.5%), TGLF 2 g kg–1 b.wt., TGLF 5 g kg–1 b.wt. and 2 satellite groups of each doses. All the animals were individually studied for the mortality, wellness parameters, body weight and histopathology for 14 days. The experiments were performed under OECD guidelines 423. Results: No mortality or any significant physiological changes in the animals were noticeable even up to high dose of 5 g kg–1 b.wt. Although, histological profiles showed toxicity indication induced by high dose of TGLF (5 g kg–1 b.wt.) in vital organs such as liver and stomach. Conclusion: Current results showed the safety nature of TGLF as natural food colorant. However, applied dose should be considered as toxicity indications are noticeable at high dose administration.