Abstract: The purpose of this study was to investigate the protective effects of ginger extract on the glycerol-induced acute kidney failure. Forty eight male Sprague-Dawley rats which divided randomly into four equal groups of 12 animals each; Group I: Normal; Group II: Received ginger extract at a dose of 400 mg kg-1 b.wt. per day by gavage; Group III: Received hypertonic glycerol (50%, v/v in sterile saline) was injected i.m. at a dose of 10 mL kg-1 and Group IV: Ginger extract, given orally at a dose of 400 mg kg-1 b.wt. per day for 30 days and injected i.m. with 10 mL kg-1 of hypertonic glycerol. The results showed that serum creatinine, Na+ and blood urea nitrogen were markedly increased in glycerol-treated rats and creatinine clearance was decreased. It is proved that ginger extract reduced their levels significantly. This study confirmed the glycerol-induced acute renal failure in terms of inflammatory infiltrate with clusters of neutrophil cells, sever hemorrhage, necrosis of some renal tubule cells together with karyolytic and pyknotic nuclei. Also, degenerated glomeruli and widening of the urinary space were noticed, deposition of intraluminal PAS positive hyaline casts and loss of the apical brush borders and decreased Ki-67 proliferation marker. The labeling index of Ki-67 in this study showed that in control group (47.3+4.6), in ginger extract group (68.7+5.16), in ARF group (35.4+4.3) and ARF treated with ginger extract group (68.9+6.2) were positively stained. However, all these adverse effects were reversed by ginger supplementation. Thus, this study suggests that ginger can be used as a nephro-protective nutrient and protect the kidney from glycerol-induced damage.