Abstract: To evaluate the effect of timolol 0.1% gel and latanoprost 0.005% on myopic regression after myopic LASIK surgery in comparison to artificial tears as placebo. The study included 90 patients with mean age of 25.7±5.7 years; 12 patients had bilateral LASIK and both eyes were included in the study. All patients underwent complete ophthalmologic examination for determination of uncorrected and corrected distant visual acuity (UDVA and CDVA), intraocular pressure (IOP), Schrimer test, central corneal pachymetry and corneal topography. These parameters were evaluated at time of inclusion and 3-monthly for 12 months. The percentage of change at the 12th month in comparison to the baseline measures, was calculated for assurance of randomization and accurate evaluation of outcome. Patients were randomly allocated into 3 equal groups: Control group received artificial tears; Timolol group received timolol maleate 0.1% gel once daily and Prostaglandin (PG) group received latanoprost 0.005% eye drops once daily. Topical therapy significantly improved UCDVA and CDVA at the end of follow-up period compared to control group and to their respective baseline measures with significantly higher percentage of improvement in timolol group compared to PG group. Control eyes showed non-significant deterioration of spherical error evaluation and extent of astigmatism at the end of follow-up compared to baseline measures, while both modalities of topical therapy significantly improved it compared to their respective preoperative measures and to control group. Timolol also, significantly improved extent of astigmatism compared to PG. The percentage of increase of corneal thickness and percentage of decrease of corneal diopter were significantly greater in control group compared to study groups. Topical therapy significantly reduced IOP in comparison to their respective pre-treatment IOP and to control group with non-significant difference between both groups. IOP lowering therapy is beneficial for amelioration of myopic regression after LASIK surgery manifested as improvement of DVA and lessening of spherical errors. Timolol 0.1% gel provided more efficient outcome compared to PG analogue and could be advocated for postoperative use.