Abstract: The study to evaluate glycaemic activity of aqueous pod extract of Acacia nilotica in normal and diabetic Wistar albino rats was conducted. Three hundred and fifty grams of the powdered sample were exhaustively extracted with distilled water using reflux method. Phytochemical investigation of the aqueous extract showed that carbohydrate, tannins, saponins, glycosides and flavonoids were present in the extract. The concentrations of Fe, K and Mn in the Acacia nilotica pod extract were within safety limit. However, the concentrations of some other elements such as Mn, Zn, Ca, Na, Cd and Cu were much lower than the acceptable levels. Elements like Pb, As and Mo were absent. There was significant (p<0.05) decrease by 13.19, 13.95, 11.73, 16.40, 28.80 and 25.46%, respectively in blood glucose level of normal rats and 14.12, 32.28, 49.67, 38.97 and 74.61% in diabetic rats treated with 400, 600 and 800 mg kg-1 of aqueous extract at 12 and 18 h post administration. Conclusively it has been deduced from the in vivo study that the extract of this plant can be used in the management of hyperglycemic condition, hence can assist in the management of disease condition such as diabetes.