Abstract: Hypercholesterolemia is a well established risk factor for cardiovascular disease and oxidative stress. Less is known about the influence of esterified phytosterols on lowering cardiovascular risk and possessing an antioxidative property. In the present study, thirty six rats divided into six groups were investigated to know the role of ω-3 fatty acid rich sterol esters (eicosapentaenoic acid-docosahexaenoic acid rich sterol ester, α-linolenic acid rich sterol ester) on hypercholesterolemia. Hypercholesterolemia was developed in the rats by adding cholesterol in the diet at the level of 1% and they were treated with two therapeutic doses (0.25, 0.5 g kg-1 b.wt. day-1) of two sitosterol esters (eicosapentaenoic acid-docosahexaenoic acid rich sterol ester, α-linolenic acid rich sterol ester). Both the sterol esters proved to be hypolipemiant but the effect of eicosapentaenoic acid-docosahexaenoic acid rich sterol ester was more pronounced than α-linolenic acid rich sterol ester. Both the sterol esters proved to be antioxidative by increasing the levels of antioxidant enzymes in the liver of the treated rats but the effect of α-linolenic acid rich sterol ester was about 40% greater than eicosapentaenoic acid-docosahexaenoic acid rich sterol ester. The overall results show that the higher dose (0.5 g kg-1 b.wt. day-1) was much effective than the lower dose.