Abstract: The different parts of Luffa echinata “Bindaal” plant are traditionally claimed and scientifically proved to be used in Liver disorders, jaundice, poisoning and migraine as antioxidant etc. but seeds part of this plant has not been explored yet. The present study was designed to investigate the free radical scavenging, anti-inflammatory and analgesic potential of methanolic extract of Luffa echinata seeds (MELE). Free radical scavenging potential of MELE was evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl method). The extract showed significant (p<0.05) free radical scavenging activity in dose dependent manner as compared with Ascorbic acid. The maximum free radical scavenging activity of MELE was found to be 82.34% at concentration of 150 μg mL-1 by the DPPH free radical scavenging method. Antioxidant effect was also investigated qualitatively using 1,1-diphenyl-2-picrylhydrazyl. The extract was further evaluated for its anti-inflammatory activity by using carrageenan-induced paw oedema in rats. Analgesic activity was evaluated by tail immersion and Hot plate methods in mice. Anti -inflammatory and analgesic activities were evaluated at dose level 50, 150 and 200 mg kg-1. The extract showed significant decrease in paw volume (60.57% reduction) and analgesic effect shown at the dose level of 200 mg kg-1 as compared to the reference drug diclofenac sodium. The MELE may be useful as a natural antioxidant in the treatment of inflammation and pain.