Abstract: The advent of the 2009 influenza A (H1N1) pandemic in April 2009 made the 2009-10 influenza season highly unusual. Public awareness of the potential seriousness of influenza was heightened by media coverage of pandemic-associated hospitalizations and deaths, especially among younger persons. The aim of our study was to investigate the attitude of Hail population towards this vaccine and possible factors associated with vaccine uptake and the effect of Hail University A/H1N1 awareness campaign conducted during the last pandemic of the disease. Design cross sectional study using self administered, anonymous questionnaires Setting Surveys at Hail University from 2nd of Nov. 2009 to 1st of Jan. 2010. A sample of 363 individuals, 277 males and 86 females from University of Hail students and personnel completed the questionnaire in the study. The overall willingness to accept pre-pandemic A/H1N1 vaccine was only (11.49%) to taking the vaccine while more than half the participants (53.44%) refused to take the vaccine. These ratios were statistically significantly different (p<0.001) of vaccination against the 2009 pandemic influenza among Hail population. The major barriers identified were fear of side effects and doubts about its efficacy. The willingness to accept pre-pandemic influenza vaccination was low and no significant effect was observed with the change in WHO alert level. Further studies are required to elucidate the root cause of the low intention to accept pre-pandemic vaccination.