Abstract: The objective of this study was to evaluate whether cryopreservation of small volume of sample (sperm+cryoprotectant) was feasible using open pulled straw and also compared the outcomes of open pulled straw and conventional straw as carrier for normal human sperm cryopreservation. Semen samples were obtained from 10 men undergoing evaluation for infertility after 3-4 days of abstinence. Washed normal sperm samples were divided into three aliquots as follows: (1) Fresh; (2) cryopreserved in open pulled straw and (3) crypreserved in conventional straw. In order to do cryopreservation of sperm in open pulled straw first washed normal sperm samples were mixed with equal volume of test yolk buffer and 12% v/v glycerol, later on 3-4 μL from the prepared mixture was loaded in each straw by using syringe. The loaded straws were plunged into liquid nitrogen and after 3 months recovered and thawed. Each straw was emptied of their fluid content in drop of 10 μL medium covered with mineral oil. Motility of vitrified-thawing sperm was assessed by using inverted microscope. The results show as percent progress motility±SD and the p<0.05 were suggested as significant. The percent progress motility±SD of fresh sperm was evaluated as follow in study groups: For fresh group was evaluated as 59.2±7.6; the value of 37.5±8.2 in cryopreserved sperm in open pulled straws group; and value of 26.3±6.4 for conventional straw group, respectively. Statistical analysis shows that the difference between cryopreserved sperm in open pulled straws and conventional straw groups is significant (p = 0.001). Because of the significant difference between cryopreserved sperm in open pulled straws and conventional straw groups, we concluded that vitrification of human sperm is feasible using open pulled straw. The results of this study shows that open pulled straw could be a good carrier for cryopreservation of small volume of normal human sperm.