Abstract: The present study was designed to examine the neuroprotective effects of post-ischemic treatment with ASA on pyramidal neurons of the hippocampal CA1 sector and spatial learning and memory. Animals were allocated to ASA (20, 40 and 80 mg kg-1) and vehicle groups. Ischemia was induced by 20 min middle cerebral artery occlusion. ASA solution was administered intraperitoneal at 30 min, 6 and 24 h after induction of ischemia. Four days after ischemia, animals were subjected to 5 days of training in the Morris water maze (MWM); 4 days with the invisible platform to test spatial learning and the 5th day without platform to test spatial memory. At the end of behavioral test rats were sacrificed and pyramidal neurons in the CA1 sector were stained with Hematoxylin and Eosin. We demonstrated that repeated injections of 20 mg kg-1 ASA (p<0.05) but not 40 and 80 mg kg-1 ASA (p = 0.989) increased percentage of spent time in the target zone across the four days post stroke test period significantly in the MWM as compared to vehicle group. But repeated injections of 20, 40 and 80 mg kg-1 of ASA after ischemia not reduced the prolongation of the escape latency significantly as compared to vehicle group (p = 0.556). In treated groups there was no significant effect on spatial memory (p = 0.987) as compared to vehicle group. Histological verification of CA1 area showed that repeated injections of 20, 40 and 80 mg kg-1 ASA at 30 min, 6 and 24 h after ischemia reduced the number of dead hippocampal neuronal cells significantly (p<0.001). Study findings show that repeated ASA injections at 30 min, 6 and 24 h after stroke onset decreases neuronal injuries following ischemia and attenuates cerebral ischemia-induced learning dysfunctions.