Abstract: This study was performed on 100 jaundiced neonates as cases and on 100 neonates without hyperbilirubinemia as controls to investigate the incidence of UTI in neonates with hyperbilirubinemia. Medical history, physical examinations and laboratory tests were done on all cases and data was analyzed by SPSS software. The urinary analysis and culture (U/A, U/C) were performed on the urinary samples which were collected by bladder catheterization under sterile condition. Of the total of 100 jaundiced neonates, 11 cases had UTI, while there was no case of UTI in neonates without jaundice. This difference in the incidence of UTI between two groups was significance (p-value = 0.001). Vesicourethral reflux was the most common finding anomaly in the neonates with UTI. Our results indicate that UTI is one of the important factors in neonatal jaundice. Therefore it is necessary to do U/A and U/C in all the neonates with hyperbilirubinemia. In addition, radiological studies such as sonography and voiding cystourethrography (VCUG) in all the neonates with UTI are necessary.