Abstract: The purpose of this study was to evaluate the effect of long-term cold exposure on the activity of Glutathione Peroxidase (GPX), Lactate Dehydrogenase (LDH) and Free Radicals (FR) production in spiny tailed lizard, Uromastyx aegyptius. Fifteen animals were randomly assigned to two groups. The experimental group underwent whole body cold exposure (8-10°C) for seven weeks period. In comparison with the control group FR production and LDH activity were increased significantly (p< 0.05) whereas GPX activity was decreased significantly (p< 0.05) under cold exposure. The regression of LDH on FR was significant (R2 = 0.67) indicting that FR affects LDH activity and there is a commonality exists between both variables. Based on the results of the present study it can be concluded that cold exposure induces acclimation in LDH activity. However the reductions of GPX reflect cold intolerance.