Abstract: The present study was performed in order to identify the organisms in poultries by culture and the PCR. This is the first step in an effort to devise strategies for control of human Campylobacters infection in Zanhedan. One hundred and sixteen samples of rectal swabs from chickens of Zahedan (south-east Iran) poultry farms were collected and cultured on Campylobacter selective agar. Subsequently, identification was carried out by biochemical tests. DNA was isolated from suspensions, which were already prepared from stool samples and purified with specific kits. The PCR was done by application of species-specific primers of C. jejuni and C. coli. The results of culture in case of 11 samples were positive out of them 8 were as C. jejuni and 3 belonged to C. Coli, whereas in case of the PCR results 27 samples were positive out of them 18 isolates belonged to C. jejuni and 9 were found as C. coli. Hence, sensitivity and specificity of the PCR were found as 100 and 84.7%, respectively, therefore it is concluded that the PCR protocol applied in this study could identify all positive samples. Furthermore, detection of the species by the PCR was exactly identical with biochemical procedures, so taking into account accuracy and rapidity of the technique, it does seem that PCR is a reliable alternative for identification of Campylobacters in stool samples of the poultries.