Abstract: To determine the seroprevalence of PVB19 infection in obstetric population, present research was conducted in Zahedan at the southeast of Iran from 2001-2002. In this case-control study, we assayed both virus specific anti-IgG and anti-IgM antibodies among 168 women with term pregnancy (controls) and 156 women with pregnancy loss at first and second trimester (cases) by ELISA method. PVB19Anti-IgG positive rate was 21.8% in pregnancy loss (case) and 20.8% in term pregnancy (control). Parvovirus B19 IgM antibody was detected in 10.3% of case and 6.5% in control. Although specific Parvovirus B19 IgG and IgM antibodies had no statistically significant difference between two groups by the age and parity, but our study showed that the prevalence of parvovirus B19 IgM antibody was higher in women with unsuccessful pregnancy. It is considerable, that high levels of IgM antibody probably support the association between PVB19 infection and unsuccess-pregnancy.