Abstract: The aim of this study was to determine the prevalence of HBV infection in barbers and then to compare with results in control group. This study was a case-control survey in a time period of 4 months in Zahedan. A total of 54 women barbers and 100 cases of women blood donors were enrolled in this study. All barbers were questioned regarding risk factors for HBV infection and duration of occupation. There was no any risk factor in blood donors. Then blood samples tested for HBsAg and anti-HBc by ELISA method (Sorin-Biomedica Kit) in Zahedan Blood Transfusion Organization. After testing on blood, samples, we observed that, prevalence of infection was higher in barbers (31.4%) than in comparison group (16%). There was a significant relation between the prevalence of infection with accidental needle pricts (p = 0.005). Also, significant difference was found between prevalence of infection and duration of occupation (p = 0.001). Present data showed that hepatitis B virus infection may constitute occupational hazards for barbers. Therefore, barbers should be made aware of the route of transmission of HBV infection and need to be screened for markers of infection on a basis and are prime candidates for immunoprophylaxis with hepatitis B vaccine.