Abstract: The effects of orally administered decoction of the dried flower of Hibiscus sabdariffa on 2, 4-dinitrophenylhydrazine-induced lipid peroxidation and reduced glucose concentration in blood, brain and liver have been examined in rabbits. Following oral administration of the decoction (200 mg kg-1 body wt.) once/day for 3 days, 2, 4-dinitrophenylhydrazine (2, 4-DNPH) was administered in saline (28 mg kg-1 body wt.) intraperitoneally. Three hours after 2, 4-DNPH administration the rabbits were sacrificed. The results show that treatment with 2, 4-DNPH alone significantly (p<0.05) reduced brain glucose when compared with the control (control 0.23±0.02 mM; 2, 4-DNPH treated: 0.12±0.03 mM). Treatment with the decoction before 2, 4-DNPH caused significant (p<0.05) increase in brain glucose relative to that of the group treated with 2, 4-DNPH alone (DNPH alone-0.12±0.03 mM; Decoction + DNPH-0.18±0.01 mM): Exposure of rabbits to 2, 4-DNPH produced significant (p<0.05) increase in lipoperoxidation in blood (28.20±4.19 units/mL x 10-7) brain (21.30±4.28 units/mL x 10-7) and liver (36.97±2.72 units/mL x 10-7) as indicated by malondialdehyde level when compared to the control (blood: 1.54±0.22; brain: 5.25±0.56; liver: 7.50±2.67 units/mL x 10-7). In rabbits treated with the decoction prior to DNPH the level of malondialdehyde was significantly (p<0.05) decreased in the blood (6.73±0.55 units/mL x 10-7) brain (6.93±0.20 units/mL x 10-7) and liver (9.37±2.14 units/mLx10-7) relative to the group treated with DNPH alone. The data obtained in this study show that the decoction of the dried flower of H. sabdariffa was able to prevent not only 2, 4-DNPH-induced decrease in brain glucose but also its lipoperoxidative effect in the blood, brain and liver in the rabbit.