Abstract: In the present study determined the relative importance of recognized etiologic agents of AVH and to compare the clinical, biochemical and demographic factors of AVH in Zahedan the capital city of the Sistan and Baluchestan province. Blood samples as well as demographic and clinical data were collected from 263 consecutive patients with AVH who were seen at Zahedan hepatitis clinic and Bu-Ali and Ali-Asghar Hospitals. Patients were tested for HbsAg, IgM Anti HAV, IgM antiHBc, IgM Anti HDV and HCV as needed. Hepatitis E was not investigated as relevant diagnostic kits were not available. A total of 263 patients with AVH were studied. 188 (75.5%) had hepatitis A, 47 (17.9%) hepatitis B, 6(2.3%) hepatitis C, 5(1.9%) hepatitis D and 17(6.6%) had hepatitis non A-D. Hepatitis A occurred most frequently in young children and patients with hepatitis B, C and D occurred generally in young adults. Mean age of patients affected by hepatitis A, were (6.1, CI 95%:5.4-6.9) B, (20.6, CI 95%:17.5-23.7) C, (20.2, CI 95%:5.7-34.7) D, (26.2, CI 95%:16.2-36.2) and Non A-D (18.7, CI 95%: 10.9-26.4). Hepatitis A patients presented to hospital or clinic earlier (p=0.01) and had lower initial serum bilirubin, ALT and AST, but had higher Alkaline Phosphatase (ALP). Who had parenterally transmitted hepatitis were more likely to have excoriation, however patients with hepatitis A were more likely to have anorexia, vomiting, fever, chill, abdominal pain and prodromal symptoms. Nearly all cases of AVH in children were due to hepatitis A, whereas hepatitis B, C and D generally occur in adults and there is some difference in clinical and laboratory findings across the etiology of AVH.