Abstract: The synthesis and spectroscopic investigations like 1H-, 13C-NMR of novel organotin (IV) esters are described. The FT IR study is successfully applied to verify the bonding mode of endo and exo status of tin (IV) of the dimeric nature of organotin (IV) compound 2. In vitro ED50, bactericidal, fungicidal, bioactivities are investigated, which indicate them significantly potential biocides. Based on spectroscopic analysis and literature evidences, the mono-and dimmer organotin (IV) esters are assigned tetrahedral and distorted octahedral cage type geometry.