Abstract: Hair and nails samples were collected from different adults women from Riyadh region, Saudi Arabia and analyzed for arsenic (As), lead (Pb) and microbial flora. The hair and nails contain higher concentrations of Pb 4.47±0.41;10.48±0.53 followed by As 0.036±0.001; 0.087±0.005, respectively. Ten genera of microorganisms were isolated from the tested samples but hair contains higher bacteria and fungi than nails. The results provide evidence of high accumulation of lead and arsenic, which might be important in dealing with metal pollution in Saudi Arabia.