Abstract: DNA polymorphism in genomic fingerprinting generated by randomly amplified primed polymorphic DNA markers (RAPD) can distinguish between strains of almost any organism. The RAPD technique which is an application of AP-PCR was applied to 25 isolates of two different Vibrio species, V. cholerae and V. harveyi with 20 different primers. Fourteen oligonucleotide primers yielded clear and reproducible bands corresponding to amplified products and were separable by agarose gel electrophoresis. All the primers used yielded multiple DNA markers with markers ranging in size from 250 - 6,000 nucleotide base pairs. The overall percentages of similarity ranged from 32.5 to 99.4 % with a mean 74.8 % for V. cholerae and 32.5 to 73.0 % with a mean 43.7 % for V. harveyi. The dendrogram produced from the RAPD fingerprint analysis showed that the V. harveyi isolates were grouped into one cluster, whereas V. cholerae isolates were grouped into 2 clusters. Results demonstrated that RAPD analysis is a simple and fast reliable technique that can clearly type Vibrio spp. and therefore can be applied for phylogenetic studies.