Abstract: The antibacterial activities of five different brands of unifloral honey from the Northern region of Bangladesh were investigated. The honeys were from Litchi (Litchi shinensis), Kadom (Anthocephalus cadmba), Til (Seasamum indicum), Plum (Zizyphus mauritiana) and Mustard (Brassica campestris) flowers. It was found that the tested unifloral honeys showed a significant antibacterial activity against the wound infecting and enteric pathogens. Among them honey from Mustard flower was more active.