Abstract: The antibacterial activities of ethyl acetate(CH3COOC2H5),methanol,(CH3OH) chloroform(CHCl3) and acetone(CH3COCH3) extracts of Helichrysum plicatum subsp. plicatum were tested in vitro against 15 different bacterial species and strains, by using the disc diffusion method. The extracts of the leaves, stem and flowers showed various inhibitory effects (7-31 mm50 μl-1 inhibition zone) but All the plant parts showed no inhibitory effect against Corynebacterium xerosis UC 9165.Therefore, due to its antibacterial activities it could be used to as a raw material for therapy purpose.