Abstract: Background and Objective: Hepatic fibrosis is a wound healing response to insults and as such affects the entire world population. In industrialized countries, the main causes of liver fibrosis include alcohol abuse, non-alcoholic steatohepatitis and chronic hepatitis virus infection which accounts for about 80% of liver fibrosis. A common denominator that is central to liver fibrosis is the activation of hepatic stellate cells, which is triggered by a plethora of signaling pathways. Liver fibrosis can progress into more severe stages, known as cirrhosis, when liver acini are substituted by nodules and further to hepatocellular carcinoma when the architecture of the liver is disfigured. This study was carried out to assess the hematological/chemical pathological and HBV DNA indices of a fibrosis liver in order to determine the efficacy of the extemporaneous polyherbal formulation. Materials and Methods: The safety and pharmacological properties of extemporaneous polyherbal preparation was evaluated. Acute toxicity study using Wistar rats revealed that oral administration of aqueous extract of homogenized herbal preparation of Allium sativum, Zingiber officinale, Curcuma longa and Moringa Oleifera was non-toxic and LD50 was found to be above 5000 mg kg–1. Microbiological examination showed that these herbal products were free of microbial contamination. Sequel to the National Agency for Food and Drug and Administration and Control (NAFDAC) guidelines on the use of herbal preparations in Nigeria, five consenting adults were given an extemporaneous herbal preparation (2000 mg kg–1) in divided dose 12 h for 3 months. The data obtained were analyzed using descriptive statistics, chi square test and independent t-test. Results: Results obtained from descriptive statistics, chi square test and independent t-test showed that there was a significant (p<0.05) improvement in aspartate aminotransferase (AST) to platelet ratio index (APRI) and hepatitis B (HBV) DNA values which were close to normal in most of the participants. Conclusion: It can succinctly be inferred that non-orthodox medications that are non-toxic and with suitable dosage forms and stability can be an alternative for the management of liver fibrosis.