Abstract: Background and Objective: Cell immobilization methods have been developed in order to improve enzymatic production. The aim of this work was to use immobilized bacteria on enzyme production to improve cell stability and to enhance the time of fermentation. Materials and Methods: The variables sodium alginate, number of cells, time of gelation, aeration and silica concentration in cells immobilization for CGTase production were evaluated. Statistical analysis of enzymatic response was performed using paired t-test (0.5%). Results: The better conditions for maximal production of CGTase (389.94 U mL–1) with immobilization of Bacillus circulans ATCC 21783 were: 3% sodium alginate; 0.2 g L–1 initial cells; 100 rpm aeration and 3% silica. There was an increase of 83.05% in enzymatic production with cell entrapment in silica. The beads cell reutilization was possible during three cycles of fermentation during 216 h. Conclusion: The enzymatic production with the beads cells reutilization provided an increase in productivity and low costs of fermentation processes. The immobilization of cells in silica beads maintains cell stability and allows the reuse of the system during CGTase production.