Abstract: Background and Objective: Currently, succinic acid fermentation process has not been successfully commercialized. Key technical problems blocking rapid advances in developing a bioprocess technology for succinic acid fermentation are of low productivity, multiple product formation and inefficient recovery of product from the fermentation system. The aim of this study was to emphasize the need to carry out the isolation, screening and identification of potential bacteria for succinic acid production. Materials and Methods: Bacterial strains were isolated from different sources in Thailand. Primary screening on selective medium plate then the fermentation process under the anaerobic condition was performed. The succinic acid was analyzed by TLC method and confirmed by HPLC method. The selected isolates were studied for phenotypic characterization, 16S rRNA gene sequence and phylogenetic analysis. Results: One hundred and fifty nine Lactic Acid Bacteria (LAB) were isolated. Only 19 succinic acid producing isolates were divided into three groups: Group I (13 isolates) were identified as Enterococcus casseliflavus (Group I-A, 2 isolates), E. durans (Group I-B, 1 isolate), E. faecium (Group I-C, 2 isolates), E. hirae (Group I-D, 7 isolates) and E. saccharolyticus (Group I-E, 1 isolate). Group II (2 isolates) were identified as Lactobacillus fermentum (Group II-A, 1 isolate) and L. oris (Group II-B, 1 isolate). Group III (4 isolates) were identified as Lactococcus formosensis (Group III-A, 1 isolate) and L. garvieae (Group III-B, 3 isolates). Enterococcus strains were capable of producing succinic acid in a range from 23.312-43.482 g L–1 while Lactobacillus and Lactococcus strains produced succinic acid in the range from 12.586-25.247 and 18.847-22.172 g L–1, respectively. The highest production of succinic acid (43.482 g L–1) was obtained from E. faecium NS13-dB1. Conclusion: Among 19 isolates, the strain Enterococcus faecium NS13-dB1 was the most effective for succinic acid production and could be a promising candidate for further applications.