Abstract: Background: Fusarium oxysporum causes vascular wilt diseases in a wide variety of economically important crops. Control of plant diseases still relies mainly on the use of synthetic fungicides, but environmental and health concerns and the development of fungicide-resistant pathogens have stimulated the search for alternative control strategies. Materials and Methods: Fusarium oxysporum isolated from the roots of diseased mung bean plans collected from Aga district, Dahahlia, Egypt (F1) recorded the highest values of wilt disease incidence (50%) in the pathogenicity test. Results: Cytokinins at concentrations of 25, 50, 100 and 200 ppm and β-sitosterol at 10–1, 10–3, 10–5 and 10–7 M slightly reduced the linear growth of F. oxysporum on PDA solid medium. In the greenhouse experiment, the β-sitosterol at 10–5 M was the most effective treatment in reducing the incidence of wilt (11.38e) in mung bean plant infected with Fusarium oxysporum . The growth parameters of mung bean plants (root length, shoot length, root fresh and dry weight, shoot fresh and dry weight and No. of leaves per plant) were significantly increased at the treatments cytokinins at the concentration 100 ppm and β-sitosterol at the concentration 10–5 M. Similarly, the photosynthetic pigments (chlorophyll a, chlorophyll b, carotonoids and total pigments) as well as the defense enzymes (peroxidase, polyphenoloxidase and catalase) activity and the content of total phenols were significantly increased by the same treatments. Also, the yield parameters (No. of pods per plant, weight of pods per plant, No. of seeds per plant and weight of seeds per plant) were significantly increased in mung bean plants non-infected and infected with Fusarium oxysporum treated with cytokinins at the concentration 100 ppm or β-sitosterol at the concentration 10–5 M. Conclusion: It is evident from the above results that, the resistance of mung bean plant to the used pathogenic fungi (Fusarium oxyspurm) was more or less improved by priming the seeds in kinetin and β-sitosterol specially in response to 100 ppm kinetin and 10–5 M β-sitosterol, these plant growth regulators could be used, as safe compounds to improve the resistance of mung bean plant to fungal pathogens.