Abstract: Contagious agalactia is an endemic disease in most of the parts of the world with the classical etiology of M. agalactiae which accounts for 90% outbreaks of contagious agalactia syndrome in goats. The disease is responsible for high economic losses due to loss in milk yield and kids/lambs because of abortions, neonatal deaths and loss of animals. It has been reported from various parts of the country including India. However, till date no specific diagnostic is available in India due to cross reactivity of mycoplasma antigens. The conventional methods of diagnosis are cumbersome and lack specificity and sensitivity. Keeping this in mind the present study was undertaken with the objective to conduct the antigenic analysis of Indian isolates of M. agalactiae by separation of proteins by SDS-PAGE and estimating their immunogenicity and diagnostic potential. SDS-PAGE revealed 24 polypeptides in Whole Cell Antigens (WCA) and Sonicated Supernatant Antigens (SSA) of both the isolates, respectively in the range of 20.89-181.97 kDa with the seven major proteins of 63.10, 60.25, 58.88, 47.86, 44.66, 33.88 and 28.84 kDa molecular weights. On immunoblotting with polyclonal rabbit serum raised against M. agalactiae RPNS 216. All the major proteins appeared immunogenic with 12-14 immunogenic polypeptides. Out of them proteins of 47.86, 44.66, 33.88 and 28.84 kDa might be of protective and diagnostic importance for M. agalactiae and could be the source for future diagnostics in India.