Abstract: The outlines of antibiotic resistance of some probiotic microorganisms were studied. This study was conducted to evaluate the antibiotic resistance of LAB strains and Bifidobacterium spp., present in Egyptian markets. Disc diffusion method and optical density was used to determine the survival percentage of bacteria. After exposed to two antibiotics, 6 selected lactobacilli and bifidobacteria were assayed above were resistant Lactobacillus lactis to tetracycline (30 μg L-1) but when these strains exposed to chloramephnicol (30 μg L-1) we found that L. acidophilus, L. casei, L. planterum and L. Lactis were sensitive but S. thermophilus and B. bifidum were not. These strains were further investigated for their safety properties including sensitivity to antibiotic in MRS broth media the results showed that L. lactis, L. acidophilus and B. bifidum strains were the most resistant to tetracycline but L. casei, S. thermophilus and B. bifidum were the most resistant strains to chloramephnicol.