Abstract: The root knot nematode (Meloidogyne spp.) is a polyphagous plant pest causing losses in a wide range of host crops. Due to environmental health concerns, the search for new microbial strains as nematode control agents is relevant. Soil samples were collected from the rhizoshere of nematode infested and non infested cucurbits plants and fungi isolated and identified using standard methods. Extraction of nematodes was done using Cobb’s sieving and decanting method and modified Baerman’s funnel technique. Cell free Fungal Culture Filtrates (FCF) were prepared from the most frequently occurring isolates and their nematicidal potentials assessed as the mean percentage mortality of nematodes at an interval of 24, 48 and 72 h. Sterile distilled water served as control. In all 18 fungal species were isolated from the rhizosphere soil of Momordica charantia control and 08 species from that of Momordica charantia infected and 09 species from Lagenaria siceraria infected. Among the nine fungi tested for nematicidal properties, Aspergillus flavus showed maximum nematode mortality (45%) after 24 h. After 48 h maximum nematode mortality was shown by Aspergillus species 2 and Trichoderma viride (68.75%). After 72 h, maximum nematode mortality was observed due to Trichoderma viride and Aspergillus flavus (93.75%). The results suggest that the various fungi associated with the rhizosphere soil of different vegetable crops could be used as a biocontrol agent of nematodes and the culture filtrates can serve as a source of novel nematicidal compound of fungal origin which is more environmental friendly.