Abstract: Traditionally important plant Trichodesma sedgwickianum and Trichodesma indicum has therapeutic utility in folk medicine having anti-inflammatory, wound healing and anticancer properties; this led us to carry out the a research based study on antimicrobial properties and identification of phytoconstituents. The antimicrobial activity has been checked against five gram positive and gram negative bacteria and three fungi. The zone of inhibition of extracts ranged from 12 to 29 mm. The MIC was found in the range of 5 to 0.625 mg mL-1. Amongst the extracts, ethanol extract of both species was more active against gram positive bacteria, S. aureus and B. subtilis whereas aqueous extract had having strong inhibitory effect against gram negative bacteria like E. coli and other organism. Phytochemically they contain steroidal, b-sitosterol and phenolics, catechin and gallic acid (HPLC). Plants have proved to be significant natural resources as effective antimicrobial and chemotherapeutic agents and offer a broad spectrum of activity with greater emphasis on preventive action.