Abstract: Macrolide-lincosamide-streptogramin-B (MLSB) are important antibiotic family for treatment of staphylococcal infections in both humans and animals. Clindamycin is widely used in veterinary medicine to treat a variety of bacterial infections. Thus, this study was conducted to investigate the incidence of MLSB resistance phenotypes among the Staphylococcus aureus isolated from animals by agar disk diffusion (D-test) method. A total of 158 coagulase positive staphylococci were isolated from different stock animals. The incidence rate of MLSB resistance phenotype was 57 (36.1%) in Methicillin-resistant and Methicillin-sensitive Staphylococcus aureus (MRSA and MSSA). Thirty two (20.2%) isolates showed constitutive resistance (cMLSB phenotype) and 12 (7.6%) isolates showed inducible clindamycin resistance (iMLSB phenotype) while the remaining thirteen (8.2%) isolates showed MS phenotype. The rate of constitutive cMLSB phenotypes resistance (32.2%) and inducible clindamycin iMLSB (10%) resistance phenotypes was high in MRSA when compare to MSSA isolates. The higher incidence of inducible clindamycin iMLSB resistance was observed in camel isolates while the lower rate was observed in sheep's isolates. Detection of erythromycin-induced clindamycin resistance in S. aureus by routine screening is necessary so that it will help in guiding therapy and therapeutic failures may be avoided.