Abstract: Staphylococcus aureus is a pathogen known to cause biomaterial-associated infections of implants and devices. Adherent Staphylococcus aureus are highly resistant to the bactericidal activity of phagocytes and they are also resistant to most antimicrobial agents. This study aimed to evaluate the antibacterial activity and the abhesive property of hydro-distillation of Vitis vinifera seeds extract against Staphylococcus aureus. The Minimum Inhibitory Concentration (MIC) of susceptibility Staphylococcus aureus, isolated from patients, to oxacillin and hydro-distilled grape seed extract were determined by microdilution method using Muller-Hinton broth. Oxacillin or grape seed extract was added either before or after bacterial adhesion in the well of micro-titer plate. Five out of 24 isolates collected from infected burns and 7 out of 8 isolates collected from infected wounds were resistant to oxacillin (MIC≥8 μg mL-1 and the MICs of grape seed extract were ranged from 1.152 to>150 μg mL-1. The growth of Staphylococcus is effectively inhibited by the extract of grape seed when the extract is added either before or after bacterial adhesion (MIC≤150 μg mL-1). It concludes that grape seed extract inhibits the growth of oxacillin-resistant Staphylococcus aureus and it exerts abhesive effect against it.