Abstract: Microorganisms like Rhizobia, Azotobacters, Azospirillum, Phosphate Solubilizing Bacteria, Mycorrhizal fungi play an important role in plant nutrition through the uptake of critical elements even from nutrient poor and hostile soils. In recent years, use of these beneficial microbes as bio-inoculants for the production of quality planting stock in forest nurseries is well known. Selection of potential isolates of microbial inoculants is essential for application as bio-fertilizer to produce quality seedlings in nurseries. In the present study, twenty three different isolates of Phosphate Solubilizing Bacteria (PSB) were isolated from teak growing areas of Andhra Pradesh, Kerala and Tamil Nadu in India. These isolates were mass cultured and inoculated to teak tissue culture raised plantlets in nursery condition to test their efficacy on the growth and biomass production of teak. It was observed that out of 23 isolates screened, the isolates KED-4 (Bacillus subtilis) and TCO-6 (Pseuodomonas fluorescens) were the most potential isolates for enhancing growth and biomass production of teak plantlets. An attempt was also made to determine the synergistic effect between these 2 isolates. The present study demonstrated that application of both the isolates together not only enhanced the growth but also increased the biomass and the quality of the plantlets.