Abstract: Seventy one bacterial isolates were obtained from the normal local habitats of Lactic Acid Bacteria (LAB) like babies buccal cavity, woman milk (two weeks after birth), animal milk (cow, goat and camel) and fermented food (pickles). Eighteen bacterial species were isolated from buccal cavity, whereas 53 species from milk and pickles samples. They were characterized and identified through physiological, biochemical tests and API 50CH kit. The isolates belonged to the genera Lactobacillus (14 strains), Pediococcus and Lactococcus (8 strains, each), Streptococcus (6 strains) and Leuconostoc (4 strains). Genus Lactobacillus includes L. plantarum, L. helveticus, L. curvatus and L. lactis while Pediococcus includes P. pentosaceus and P. acidilactici. Genus Lactococcus was presented by L. lactis whereas genus Streptococcus was presented by S. mitis, S. sobrinus, S. salivarius and S. ratius. Genus Leuconostoc includes L. mesenteroides and L. lactis. Screening of all LAB isolates (71) for antimicrobial activity revealed that 40 bacterial isolates showed antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans. The highest activity was achieved by Streptococcus salivarius, Lactococcus lactis, Pediococcus pentosaceus and Lactobacillus plantarum. No activity was observed against methicillin resistant Staphylococcus aureus (MRSA), Aspergillus niger and Fusarium oxysporum.