Abstract: Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI in patients with diabetes mellitus are reported with asymptomatic bacteriuria. To ensure appropriate therapy, current knowledge of the organisms that cause UTI and their antibiotic susceptibility is mandatory. This study focused on the frequency of uropathogens and their antibiotic susceptibility in different gender of diabetic patients. Biochemical characterization of uropathogens revealed the prevalence of gram negative organisms and E. coli as the predominant isolate. Among the antibiotics tested, trimethoprim was found to be effective for empirical treatment of UTI and has covered the majority of urinary pathogens followed by nalidixic acid, chloramphenicol and kanamycin. Most of the isolates were resistant to oxytetracycline which is more frequently prescribed and indicates that increased consumption of a particular antibiotic can be a pathway to its resistance by the uropathogens. Conclusively, resistance rates among common uropathogens continue to evolve and appear to be increasing too many commonly used antimicrobial agents and a continued surveillance of resistance rates among uropathogens is needed to ensure appropriate recommendations for the treatment of the urinary tract infections.