Abstract: The ecofriendly synthesis of nanoparticles through various biological means, helps to explore various herbs for their ability to synthesis nanoparticles. In this study, soap nuts-which are the part and parcel in daily life of Indians from several centuries, are used to synthesize silver nanoparticles. The synthesized nanoparticles were characterized under UV-Visible spectrophotometer, SEM, TEM and EDX. The antibacterial activity of silver nanoparticles was performed on various gram positive and gram negative bacteria. The silver nanoparticles showed more inhibitory activity on pathogenic gram positive than gram negative bacteria.