Abstract: Choline dehydrogenase catalyzes the oxidation of choline to glycine betaine via betaine aldehyde in glycine betaine biosynthesis. Glycine betaine is a compatible solute, able to restore and maintain osmotic balance of living cells under stress. In this study, choline dehydrogenase (betA) gene encoding for glycine betaine biosynthesis in Escherichia coli isolated from salted shark (Scoliodon sp.) was cloned and sequenced. The betA gene sequence reported in this study contains several base substitutions with that of reported sequences in GenBank, resulting in the altered amino acid sequences of the translated proteins.