Abstract: This study was done to screen seafoods in Lagos for the presence of Vibrio and Aeromonas spp. that have multi-drug resistance plasmid. The samples were cultured on Thiosulphate Citrate Bilesalt Sucrose agar (TCBS) agar after enrichment in Alkaline Peptone Water (APW). Using API 20E system and complementary biochemical test, 83 VIbrio species and 3 Aeromonas hydrophila were identified from the samples. The Vibrio species encountered comprised of V. cholerae (3) V. parahaemolyticus (5) V. mimicus (16) V. alginolyticus (37) and V. harveyi (12), V. vulnificus (10) and Aeromonas hydrophila (3). Antimicrobial susceptibility carried out using standard procedures revealed that all the 86 (100%) isolates were resistant to Augmentin, Chloramphenicol and Amoxicillin (Amx-Au-Chl). Ten (11.6%) of the isolates had multiple resistance to all the 10 antibiotics tested: Gentamicin, Nitrofuratoin, Tetracycline, Chloramphenicol, Amoxicillin, Ofloxacin, Cotrimoxazole, Augmentin, Ciprofloxacin, Ceftriazone (Amx-Aug-Chl-Nit-Cip-Tet-Ofl-Gen-Cot-Cro). Plasmid characterization revealed that only four (4.7%) of the Eighty-six isolates haboured plasmid DNA. These were two strains of V. alginolyticus with plasmids of molecular weight of 25.0 and 9.416 Kbp each and two strains of V. mimicus with plasmids of molecular weight 4.361 Kbp each. None of the Aeromonas specie haboured plasmids. This shows that antibiotic resistance in Vibrio and Aeromonas species in this environment is not plasmid mediated.