Abstract: The aim of this present study was to identify possible lead poisoning prevention strategies. Six fresh and five spoilt paint samples were collected from a reputable paint industry in Lagos, Nigeria. The microbial population count in the fresh paint samples monitored at two week interval in a ten-month study ranged from 1.6x101-5.5x103 cfu mL-1 while that of spoilt paint samples ranged from 1.0x107-3.4x1010 cfu mL-1 for bacteria and fungi, respectively. The isolated bacteria were identified as Bacillus polymyxa, B. brevis, B. laterosporus, Proteus mirabilis, Escherichia coli, Lactobacillus gasseri and L. brevis based on isolate’s phenotypic profiles using the Analytical Profile Index (API) ID 32E test systems. The fungal isolates were Aspergillus niger, A. flavus and Penicillium citrinum. In addition to these organisms, a strain of Pseudomonas aeruginosa which was observed to possess plasmids with molecular weights ranging from 0.030 to 0.112 kb was isolated from the spoilt paint samples. Atomic Absorption Spectrophotometric (AAS) analysis revealed that the spoilt paint samples had higher concentrations of heavy metals ranging from 2.0-5.3, 2.5-5.8 and 3.0-5.0 mg kg-1 while the fresh paint samples had 2.0-2.5, 1.0-3.0 and 2.0-3.1 mg kg-1 for Pb, Cu and Mn, respectively. Subsequent to curing experiment with 0.002% (v/v) Sodium Dodecyl Sulphate (SDS), Pseudomonas aeruginosa was observed to have lost the two existing plasmids. The incorporation of SDS (which successfully cured plasmids in paint spoilage organism) in paint formulation, suggests an environmental health risk prevention strategy because it will reduce spoilage. Also, the use of lead in providing durability in paints may be prevented.