Abstract: The aim of the present study was to evaluate antibacterial and antioxidant activity of different solvent extracts of Mangifera indica L. seeds. The seeds of Mangifera indica L. were successively extracted in petroleum ether, chloroform, ethyl acetate, acetone and methanol. All obtained extracts were evaluated for antimicrobial and free radical scavenging (DPPH) activity. The in vitro antimicrobial activity was done by agar disc diffusion method at a concentration of 600 μg disc-1 against 5 Gram-positive bacteria, 7 Gram-negative bacteria and 3 fungal strains. The antioxidant potential was evaluated using scavenging of DPPH radical. Maximum antibacterial activity was shown by methanol extract followed by acetone extract. Acetone (IC50 = 11 μg mL-1) and methanol (IC50 = 12 μg mL-1) extract also showed DPPH scavenging activity which was comparable with that of standard ascorbic acid (IC50 = 11.4 μg mL-1). This study has highlighted the potentiality of antibacterial and antioxidant properties of M. indica seeds.