Abstract: The effect of yeast extract supplementation on bacterial curdlan production using a medium containing corn syrup and the corn-based ethanol coproduct condensed corn distillers solubles was determined. Curdlan was produced by Agrobacterium sp. ATCC 31749 on a medium containing selected solubles concentrations as a source of nitrogen and corn syrup as a carbon source. The presence of yeast extract in the medium was found to enhance bacterial curdlan production at all three concentrations of solubles tested after 120 h of growth. Bacterial biomass production was also noted to be higher after 120 h when the cells were supplemented with yeast extract. It was concluded that the observed increase in curdlan production by the yeast extract-supplemented ATCC 31749 cells was due to the yeast extract stimulating biomass formation.