Abstract: Sixteen soil samples were collected from four different plots of cassava plantation and analyzed for their microbiological and physicochemical characteristics. A total of twelve microorganisms were isolated consisting four bacteria, seven fungi and one actinomyces. The bacteria were Bacillus cereus, B. megaterium, B. polymyxa, B. subtilis, while the fungi included Aspergillus flavus, A. fumigatus, A. niger, A. repens, Botrytis cinerea, Neurospora sitophila, Varicosporium elodea and Actionmyces reticuli. Plot A recorded the highest microbial counts of 7.95H105 cfu gG1 and 4.18H103 sfu gG1 for bacteria and fungi respectively, while the control (uncultivated soils) had the lowest microbial counts of 1.73H105 cfu gG1 and 1.50H103 sfu gG1 for bacteria and fungi, respectively. Actinomycetes were found only in plots B and D. The colour of the soils varied from black, brownish black, yellowish brown to complete brown, while the texture ranged from very coarse, through granular to very fine. Chemical analysis revealed pH range of 5.67 to 6.70, moisture content of 10.08 to 14.70%, organic matter content of 8.48 to 13.90% oxidizable organic carbon of 0.11 to 0.41% and ash content of 8.37 to 13.40%. Mineral analysis showed the presence of N, P, K+, Na+, Ca2+, Mg2+ ppm in varying proportions. Therefore, cassava cultivated soils has not suffered any significant depletion of nutrients.