Abstract: While screening the actinomycetes for bioactive metabolites, Streptomyces purpeofuscus was isolated from laterite soil. An attempt was made to screen S. purpeofuscus for the production of antimicrobial metabolites. The cell growth as well as antimicrobial metabolite production was studied on different culture media. Secondary metabolites from the strain were active against bacteria like Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus as well as fungi including Aspergillus flavus, A. niger and Penicillium citrinum.