Abstract: The aim of present study was to investigate the seroprevalence of brucellosis among cattle and cattle-owners at selected area of Khartoum state. This research was carried out in Gubul Awliaa area farms, out of 175 unvaccinated cattle 84 (48%) were shown positive agglutination reaction towards Br. melitensis while 44 (26.4%) showed positive reaction toward Br. abortus. Mix infection was also reported. The highest incidence of infected animals and mix infection were recorded in cows with more than 10 years old. Cows milking between 6-10 times showed highest incidence of the infection. 33.1% of positive Br. melitensis cattle showed titre of 160 while 24.6% of the positive Br. abortus showed titre of 160. The Ring Milk Test (RMT) was positive in 80% of examined milk samples. On the other hand 40% from examined milker-sera showed positive agglutination results with Br. melitensis with titre of 160.