Abstract: The antimicrobial activity of three types of Bees honey (Sidir, Sunflower and Sunut) were examined against (Staphylococcus aureus, Escherichia coli, Klebsiella aerugenes, Pseudomonas aeruginosa and Candida albicans) at concentration 100, 75, 50 and 25%. Sidir honey showed antimicrobial activity against Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa and Candida albicans, the zone of inhibition range between (9-50 mm), while the E. coli showed markedly resistance towards all concentrations used. Sunflower honey showed markedly sensitivity towards E. coli, Staphylococcus aureus and Klebsiella aerogenes, the inhibitions zone were between (15-50 mm), while both Candida albicans and Pseudomonas aeruginosa showed clearly resistance towards all concentrations used. Sunut honey showed antimicrobial activity toward Staphylococcus aureus, E. coli, Klebsiella aerogenes and Candida albicans, the inhibition zone range between (10-42 mm), while Pseudomonas aeruginosa showed resistance toward all concentrations used. The antibacterial activity was compared with Gentamicin and Tetracycline, while the antifungal activity was compared with Nystatin.